Application

Tace Inhibitor Screening Assay Kit has been used in TACE (TNFa converting enzyme) activity assays.

Features and Benefits

Compatible with high-throughput handling systems.

General description

Tumor necrosis factor-a converting enzyme (TACE; ADAM17) is a member of the ADAM (A Disintegrin And Metalloprotease) protein family implicated in cell-cell and cell-matrix interactions. TACE activates Notch signaling in the epidermal cells and has a critical function in the maintenance of barrier immunity. It is also involved in neurogenesis and muscle development.

Principle

The TACE Inhibitor Screening Assay Kit is a rapid, simple and sensitive assay that is suitable for high throughput screening of TACE inhibitors. TACE hydrolyzes a FRET-tagged substrate to release a fluorescent product (λ_ex = 318/ λ_em = 449 nm), proportional to the enzymatic activity present.

Suitability

Suitable for the screening of inhibitors of TACE.